| http://dbpedia.org/ontology/abstract
|
Terminal amine isotopic labeling of substr … Terminal amine isotopic labeling of substrates (TAILS) is a method in quantitative proteomics that identifies the protein content of samples based on N-terminal fragments of each protein (N-terminal peptides) and detects differences in protein abundance among samples. Like other methods based on N-terminal peptides, this assay uses trypsin to break proteins into fragments and separates the N-terminal peptides (the fragments containing the N-termini of the original proteins) from the other fragments (internal tryptic peptides). TAILS isolates the N-terminal peptides by identifying and removing the internal tryptic peptides. This negative selection allows the TAILS method to detect all N-termini in the given samples. Alternative methods that rely on the free amino group of the N-terminus to identify the N-terminal peptides cannot detect some N-termini because they are "naturally blocked" (i.e. the natural protein does not have a free amino group). The TAILS method has a number of applications including the identification of new substrates and proteases (including those that have an unknown and broad specificity) and as a way to define the termini of proteins that enables protein annotation. TAILS can also be used to link proteases with a variety of defined biological pathways in diseases such as cancer, in order to gain a clearer understanding of the substrates and proteases involved in the disease state.d proteases involved in the disease state.
|
| http://dbpedia.org/ontology/thumbnail
|
http://commons.wikimedia.org/wiki/Special:FilePath/TAILS_overview_picture.jpg?width=300 +
|
| http://dbpedia.org/ontology/wikiPageID
|
34930324
|
| http://dbpedia.org/ontology/wikiPageLength
|
15497
|
| http://dbpedia.org/ontology/wikiPageRevisionID
|
1046244536
|
| http://dbpedia.org/ontology/wikiPageWikiLink
|
http://dbpedia.org/resource/Tandem_mass_spectrometry +
, http://dbpedia.org/resource/Quantitative_proteomics +
, http://dbpedia.org/resource/Elution +
, http://dbpedia.org/resource/Multiplex_%28assay%29 +
, http://dbpedia.org/resource/Cancer +
, http://dbpedia.org/resource/Ultrafiltration +
, http://dbpedia.org/resource/Substrate_%28biochemistry%29 +
, http://dbpedia.org/resource/Proteolysis +
, http://dbpedia.org/resource/SILAC +
, http://dbpedia.org/resource/In_vivo +
, http://dbpedia.org/resource/ITRAQ +
, http://dbpedia.org/resource/Protein +
, http://dbpedia.org/resource/University_of_British_Columbia +
, http://dbpedia.org/resource/Peptide +
, http://dbpedia.org/resource/Bioinformatics +
, http://dbpedia.org/resource/Peptides +
, http://dbpedia.org/resource/Category:Mass_spectrometry +
, http://dbpedia.org/resource/Isobaric_tag_for_relative_and_absolute_quantification +
, http://dbpedia.org/resource/Proteases +
, http://dbpedia.org/resource/N-terminus +
, http://dbpedia.org/resource/Formaldehyde +
, http://dbpedia.org/resource/Ex_vivo +
, http://dbpedia.org/resource/Category:Proteomics +
, http://dbpedia.org/resource/Metabolic +
, http://dbpedia.org/resource/Trypsin +
, http://dbpedia.org/resource/Mass_spectrometry +
, http://dbpedia.org/resource/Isotope +
|
| http://dbpedia.org/property/alt
|
Terminal amine isotopic labeling of substrates overview
|
| http://dbpedia.org/property/annotColor
|
red
|
| http://dbpedia.org/property/annotFontWeight
|
bold
|
| http://dbpedia.org/property/caption
|
TAILS Procedure Overview. Numbers in red identify steps in the text.
|
| http://dbpedia.org/property/date
|
August 2014
|
| http://dbpedia.org/property/imageWidth
|
498
|
| http://dbpedia.org/property/reason
|
why does lack of independence mean they cannot be averaged and why is this bad?
|
| http://dbpedia.org/property/wikiPageUsesTemplate
|
http://dbpedia.org/resource/Template:Chem_name +
, http://dbpedia.org/resource/Template:Annotation +
, http://dbpedia.org/resource/Template:Reflist +
, http://dbpedia.org/resource/Template:Clarify +
, http://dbpedia.org/resource/Template:Annotated_image +
|
| http://purl.org/dc/terms/subject
|
http://dbpedia.org/resource/Category:Mass_spectrometry +
, http://dbpedia.org/resource/Category:Proteomics +
|
| http://purl.org/linguistics/gold/hypernym
|
http://dbpedia.org/resource/Method +
|
| http://www.w3.org/ns/prov#wasDerivedFrom
|
http://en.wikipedia.org/wiki/Terminal_amine_isotopic_labeling_of_substrates?oldid=1046244536&ns=0 +
|
| http://xmlns.com/foaf/0.1/depiction
|
http://commons.wikimedia.org/wiki/Special:FilePath/TAILS_overview_picture.jpg +
|
| http://xmlns.com/foaf/0.1/isPrimaryTopicOf
|
http://en.wikipedia.org/wiki/Terminal_amine_isotopic_labeling_of_substrates +
|
| owl:sameAs |
https://global.dbpedia.org/id/4vLiz +
, http://rdf.freebase.com/ns/m.0j440vn +
, http://www.wikidata.org/entity/Q7702707 +
, http://dbpedia.org/resource/Terminal_amine_isotopic_labeling_of_substrates +
|
| rdf:type |
http://dbpedia.org/ontology/Software +
|
| rdfs:comment |
Terminal amine isotopic labeling of substr … Terminal amine isotopic labeling of substrates (TAILS) is a method in quantitative proteomics that identifies the protein content of samples based on N-terminal fragments of each protein (N-terminal peptides) and detects differences in protein abundance among samples.rences in protein abundance among samples.
|
| rdfs:label |
Terminal amine isotopic labeling of substrates
|
