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Structure-Based Assignment (SBA) is a tech … Structure-Based Assignment (SBA) is a technique to accelerate the resonance assignment which is a key bottleneck of NMR (Nuclear magnetic resonance) structural biology. A homologous (similar) protein is used as a template to the target protein in SBA. This template protein provides prior structural information about the target protein and leads to faster resonance assignment . By analogy, in X-ray Crystallography, the molecular replacement technique allows solution of the crystallographic phase problem when a homologous structural model isknown, thereby facilitating rapid structure determination. Some of the SBA algorithmsare CAP which is an RNA assignment algorithm which performs an exhaustive search overall permutations, MARS which is a program for robust automatic backbone assignment and Nuclear Vector Replacement (NVR) which is a molecular replacement like approach for SBA of resonances andsparse Nuclear Overhauser Effect (NOE)'s.dsparse Nuclear Overhauser Effect (NOE)'s.
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Structure-Based Assignment (SBA) is a tech … Structure-Based Assignment (SBA) is a technique to accelerate the resonance assignment which is a key bottleneck of NMR (Nuclear magnetic resonance) structural biology. A homologous (similar) protein is used as a template to the target protein in SBA. This template protein provides prior structural information about the target protein and leads to faster resonance assignment . By analogy, in X-ray Crystallography, the molecular replacement technique allows solution of the crystallographic phase problem when a homologous structural model isknown, thereby facilitating rapid structure determination. Some of the SBA algorithmsare CAP which is an RNA assignment algorithm which performs an exhaustive search overall permutations, MARS which is a program for robust automatic backbone assignment afor robust automatic backbone assignment a
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Structure-based assignment
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