http://dbpedia.org/ontology/abstract
|
The Strep-tag system is a method which all … The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin, and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions, it is especially suited for generation of functional proteins.ted for generation of functional proteins.
|
http://dbpedia.org/ontology/wikiPageExternalLink
|
http://www.strep-tag.com +
|
http://dbpedia.org/ontology/wikiPageID
|
22810498
|
http://dbpedia.org/ontology/wikiPageLength
|
7943
|
http://dbpedia.org/ontology/wikiPageRevisionID
|
1113471006
|
http://dbpedia.org/ontology/wikiPageWikiLink
|
http://dbpedia.org/resource/Optical_tweezers +
, http://dbpedia.org/resource/Chemically_inert +
, http://dbpedia.org/resource/FLAG-tag +
, http://dbpedia.org/resource/Category:Protein_methods +
, http://dbpedia.org/resource/Peptide +
, http://dbpedia.org/resource/Proline +
, http://dbpedia.org/resource/Insect +
, http://dbpedia.org/resource/Horseradish_peroxidase +
, http://dbpedia.org/resource/Tryptophan +
, http://dbpedia.org/resource/Histidine +
, http://dbpedia.org/resource/Single-molecule_experiment +
, http://dbpedia.org/resource/Biotin +
, http://dbpedia.org/resource/Alkaline_phosphatase +
, http://dbpedia.org/resource/Gln +
, http://dbpedia.org/resource/Western_blot +
, http://dbpedia.org/resource/Affinity_chromatography +
, http://dbpedia.org/resource/Immunohistochemistry +
, http://dbpedia.org/resource/Serine +
, http://dbpedia.org/resource/Escherichia_coli +
, http://dbpedia.org/resource/Amino_acids +
, http://dbpedia.org/resource/Category:Laboratory_techniques +
, http://dbpedia.org/resource/Immunocytochemistry +
, http://dbpedia.org/resource/Lysine +
, http://dbpedia.org/resource/Streptamer +
, http://dbpedia.org/resource/Yeast +
, http://dbpedia.org/resource/Category:Molecular_biology_techniques +
, http://dbpedia.org/resource/Recombinant_DNA +
, http://dbpedia.org/resource/Green_fluorescent_protein +
, http://dbpedia.org/resource/Glutamic_acid +
, http://dbpedia.org/resource/Cell_lysate +
, http://dbpedia.org/resource/Expression_clones +
, http://dbpedia.org/resource/Colony_blot +
, http://dbpedia.org/resource/Streptavidin +
, http://dbpedia.org/resource/Desthiobiotin +
, http://dbpedia.org/resource/Azo_dye +
, http://dbpedia.org/resource/Streptomyces_avidinii +
, http://dbpedia.org/resource/Category:Biochemistry +
, http://dbpedia.org/resource/Phenylalanine +
, http://dbpedia.org/resource/Protein_tag +
, http://dbpedia.org/resource/CDNA +
, http://dbpedia.org/resource/His-tag +
, http://dbpedia.org/resource/ELISA +
, http://dbpedia.org/resource/Dot_blot +
, http://dbpedia.org/resource/Molecular_biology +
, http://dbpedia.org/resource/SpyCatcher +
, http://dbpedia.org/resource/Biotechnology +
, http://dbpedia.org/resource/Category:Biotechnology +
, http://dbpedia.org/resource/Proteomics +
, http://dbpedia.org/resource/Protein +
, http://dbpedia.org/resource/Atomic_force_microscope +
, http://dbpedia.org/resource/Mammalian +
, http://dbpedia.org/resource/Buffer_solution +
, http://dbpedia.org/resource/Gene +
|
http://dbpedia.org/property/wikiPageUsesTemplate
|
http://dbpedia.org/resource/Template:More_citations_needed +
, http://dbpedia.org/resource/Template:Protein_tag +
, http://dbpedia.org/resource/Template:Reflist +
|
http://purl.org/dc/terms/subject
|
http://dbpedia.org/resource/Category:Biochemistry +
, http://dbpedia.org/resource/Category:Laboratory_techniques +
, http://dbpedia.org/resource/Category:Molecular_biology_techniques +
, http://dbpedia.org/resource/Category:Protein_methods +
, http://dbpedia.org/resource/Category:Biotechnology +
|
http://purl.org/linguistics/gold/hypernym
|
http://dbpedia.org/resource/Method +
|
http://www.w3.org/ns/prov#wasDerivedFrom
|
http://en.wikipedia.org/wiki/Strep-tag?oldid=1113471006&ns=0 +
|
http://xmlns.com/foaf/0.1/isPrimaryTopicOf
|
http://en.wikipedia.org/wiki/Strep-tag +
|
owl:sameAs |
https://global.dbpedia.org/id/4vytp +
, http://www.wikidata.org/entity/Q7623282 +
, http://yago-knowledge.org/resource/Strep-tag +
, http://dbpedia.org/resource/Strep-tag +
, http://rdf.freebase.com/ns/m.063zv8b +
|
rdf:type |
http://dbpedia.org/class/yago/Ability105616246 +
, http://dbpedia.org/class/yago/Cognition100023271 +
, http://dbpedia.org/class/yago/Technique105665146 +
, http://dbpedia.org/class/yago/Abstraction100002137 +
, http://dbpedia.org/class/yago/Method105660268 +
, http://dbpedia.org/class/yago/Know-how105616786 +
, http://dbpedia.org/class/yago/PsychologicalFeature100023100 +
, http://dbpedia.org/class/yago/WikicatLaboratoryTechniques +
, http://dbpedia.org/ontology/Software +
, http://dbpedia.org/class/yago/WikicatMolecularBiologyTechniques +
, http://dbpedia.org/class/yago/WikicatProteinMethods +
|
rdfs:comment |
The Strep-tag system is a method which all … The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin, and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions, it is especially suited for generation of functional proteins.ted for generation of functional proteins.
|
rdfs:label |
Strep-tag
|