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Selective microfluidics-based ligand enric … Selective microfluidics-based ligand enrichment followed by sequencing (SMiLE-seq) is a technique developed for the rapid identification of DNA binding specificities and affinities of full length monomeric and dimeric transcription factors in a fast and semi-high-throughput fashion. SMiLE-seq works by loading in vitro transcribed and translated “bait” transcription factors into a microfluidic device in combination with DNA molecules. Bound transcription factor-DNA complexes are then isolated from the device, which is followed by sequencing and sequence data analysis to characterize binding motifs. Specialized software is used to determine the DNA binding properties of monomeric or dimeric transcription factors to help predict their in vivo DNA binding activity. SMiLE-seq combines three critical functions that makes it unique from existing techniques: (1) the use of capillary pumps to optimize the loading of samples, (2) trapping molecular interactions on the surface of the microfluidic device through immunocapture of target transcription factors, (3) enabling the selection of DNA that is specifically bound to transcription factors from a pool of random DNA sequences.ctors from a pool of random DNA sequences.
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| rdfs:comment |
Selective microfluidics-based ligand enric … Selective microfluidics-based ligand enrichment followed by sequencing (SMiLE-seq) is a technique developed for the rapid identification of DNA binding specificities and affinities of full length monomeric and dimeric transcription factors in a fast and semi-high-throughput fashion.n a fast and semi-high-throughput fashion.
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SMiLE-Seq
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