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A Hollow fiber bioreactor is a 3 dimension … A Hollow fiber bioreactor is a 3 dimensional cell culturing system based on hollow fibers, which are small, semi-permeable capillary membranes arranged in parallel array with a typical molecular weight cut-off (MWCO) range of 10-30 kDa. These hollow fiber membranes are often bundled and housed within tubular polycarbonate shells to create hollow fiber bioreactor cartridges. Within the cartridges, which are also fitted with inlet and outlet ports, are two compartments: the intracapillary (IC) space within the hollow fibers, and the extracapillary (EC) space surrounding the hollow fibers. Cells are seeded into the EC space of the hollow fiber bioreactor and expand there. Cell culture medium is pumped through the IC space and delivers oxygen and nutrients to the cells via hollow fiber membrane perfusion. As the cells expand, their waste products and CO2 also perfuse the hollow fiber membranes and are carried away by the pumping of medium through the IC space. As waste products build up due to increased cell mass, the rate of medium flow can also be increased so that cell growth is not inhibited by waste product toxicity. Because thousands of hollow fibers may be packed into a single hollow fiber bioreactor, they increase the surface area of the cartridge considerably. As a result, cells can fill up the EC space to densities >108 cells/ml. However, the cartridge itself takes up a very small volume (oftentimes the volume of a 12-oz soda can). The fact that hollow fiber bioreactors are very small and yet enable incredibly high cell densities has led to their development for both research and commercial applications, including monoclonal antibody and influenza vaccine production. Likewise, because hollow fiber bioreactors use up significantly less medium and growth factors than traditional cell culture methods such as , they offer a significant cost savings. Finally, hollow fiber bioreactors are sold as single-use disposables, resulting in significant time savings for laboratory staff and technicians.ings for laboratory staff and technicians.
, Um biorreator de fibra oca (HFB) é um sist … Um biorreator de fibra oca (HFB) é um sistema tridimensional de cultura de células com perfusão contínua de alta densidade que oferece um ambiente semelhante ao in vivo através da utilização de fibras porosas que mimetizam os capilares sanguíneos. Seu mecanismo consiste em um feixe de tubos porosos semipermeáveis (fibras ocas) dispostos paralelamente, contidos em um cilindro externo. Entre as fibras e o cilindro fica o espaço extracapilar (EEC), onde usualmente ficam retidas as células. No interior das fibras, no chamado espaço intracapilar, ocorre o escoamento do meio de cultura, no qual os nutrientes ali contidos podem se difundir através dos poros das fibras até o EEC. Ademais, os dejetos metabólicos gerados pelas células podem retornar ao interior das fibras e seguir juntamente com o fluxo do meio. A cultura de células animais à base de fibra oca foi desenvolvida pela primeira vez por Richard Knazek no National Institutes of Health (NIH) em 1972 porém esse tipo de biorreator só atingiu seu pico de popularidade entre os anos 1970 a 1980, quando foram empregados na fabricação de anticorpos monoclonais.s na fabricação de anticorpos monoclonais.
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A Hollow fiber bioreactor is a 3 dimension … A Hollow fiber bioreactor is a 3 dimensional cell culturing system based on hollow fibers, which are small, semi-permeable capillary membranes arranged in parallel array with a typical molecular weight cut-off (MWCO) range of 10-30 kDa. These hollow fiber membranes are often bundled and housed within tubular polycarbonate shells to create hollow fiber bioreactor cartridges. Within the cartridges, which are also fitted with inlet and outlet ports, are two compartments: the intracapillary (IC) space within the hollow fibers, and the extracapillary (EC) space surrounding the hollow fibers. (EC) space surrounding the hollow fibers.
, Um biorreator de fibra oca (HFB) é um sist … Um biorreator de fibra oca (HFB) é um sistema tridimensional de cultura de células com perfusão contínua de alta densidade que oferece um ambiente semelhante ao in vivo através da utilização de fibras porosas que mimetizam os capilares sanguíneos. Seu mecanismo consiste em um feixe de tubos porosos semipermeáveis (fibras ocas) dispostos paralelamente, contidos em um cilindro externo. Entre as fibras e o cilindro fica o espaço extracapilar (EEC), onde usualmente ficam retidas as células. No interior das fibras, no chamado espaço intracapilar, ocorre o escoamento do meio de cultura, no qual os nutrientes ali contidos podem se difundir através dos poros das fibras até o EEC. Ademais, os dejetos metabólicos gerados pelas células podem retornar ao interior das fibras e seguir juntamente com o fior das fibras e seguir juntamente com o f
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Biorreator de fibra oca
, Hollow fiber bioreactor
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