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Si definiscono glicosintasi una specifica … Si definiscono glicosintasi una specifica classe di glicosidasi progettate tramite ingegneria genetica (attraverso apposite mutazioni sul sito attivo) per catalizzare la formazione di un legame glicosidico, al fine di produrre oligosaccaridi non idrolizzabili a partire da monosaccaridi. Attraverso le glicosintasi è dunque possibile produrre elevate quantità di oligosaccaridi senza che essi vengano degradati. Le glicosintasi derivano dagli enzimi glicosidasi, che catalizzano l'idrolisi dei legami glicosidici. Di solito vengono prodotte grazie alla mutazione di un amminoacido nucleofilo del sito attivo (solitamente un aspartato o glutammato), che viene trasformato in un amminoacido più piccolo e non nucleofilo (solitamente alanina o glicina).ucleofilo (solitamente alanina o glicina).
, The term glycosynthase refers to a class o … The term glycosynthase refers to a class of proteins that have been engineered to catalyze the formation of a glycosidic bond. Glycosynthase are derived from glycosidase enzymes, which catalyze the hydrolysis of glycosidic bonds. They were traditionally formed from retaining glycosidase by mutating the active site nucleophilic amino acid (usually an aspartate or glutamate) to a small non-nucleophilic amino acid (usually alanine or glycine). More modern approaches use directed evolution to screen for amino acid substitutions that enhance glycosynthase activity.tions that enhance glycosynthase activity.
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| rdfs:comment |
Si definiscono glicosintasi una specifica … Si definiscono glicosintasi una specifica classe di glicosidasi progettate tramite ingegneria genetica (attraverso apposite mutazioni sul sito attivo) per catalizzare la formazione di un legame glicosidico, al fine di produrre oligosaccaridi non idrolizzabili a partire da monosaccaridi. Attraverso le glicosintasi è dunque possibile produrre elevate quantità di oligosaccaridi senza che essi vengano degradati. Le glicosintasi derivano dagli enzimi glicosidasi, che catalizzano l'idrolisi dei legami glicosidici. Di solito vengono prodotte grazie alla mutazione di un amminoacido nucleofilo del sito attivo (solitamente un aspartato o glutammato), che viene trasformato in un amminoacido più piccolo e non nucleofilo (solitamente alanina o glicina).ucleofilo (solitamente alanina o glicina).
, The term glycosynthase refers to a class o … The term glycosynthase refers to a class of proteins that have been engineered to catalyze the formation of a glycosidic bond. Glycosynthase are derived from glycosidase enzymes, which catalyze the hydrolysis of glycosidic bonds. They were traditionally formed from retaining glycosidase by mutating the active site nucleophilic amino acid (usually an aspartate or glutamate) to a small non-nucleophilic amino acid (usually alanine or glycine). More modern approaches use directed evolution to screen for amino acid substitutions that enhance glycosynthase activity.tions that enhance glycosynthase activity.
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| rdfs:label |
Glycosynthase
, Glicosintasi
|
