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Fluorescein arsenical helix binder, kurz F … Fluorescein arsenical helix binder, kurz FlAsH-EDT2, ist eine vom Fluoreszenzfarbstoff Fluorescein abgeleitete arsenhaltige chemische Verbindung, die in Peptiden und Proteinen selektiv an bestimmte Strukturen (sog. Tetracysteinsequenzen) binden kann. Nach Bindung an seine Zielstruktur und einer Anregung mit kurzwelligem Licht zeigt FlAsH eine Fluoreszenz. FlAsH-EDT2 ist daher als Protein-Tag zur selektiven Markierung und Identifizierung von Proteinen geeignet. Ebenso wird dieser Farbstoff als ein Fluorophor für Förster-Resonanzenergietransfers (FRET) verwendet. FlAsH-EDT2 kann sowohl in die Gruppe der Triphenylmethanfarbstoffe, als auch der Xanthenfarbstoffe eingeordnet werden. der Xanthenfarbstoffe eingeordnet werden.
, FlAsH-EDT2 is an organoarsenic compound wi … FlAsH-EDT2 is an organoarsenic compound with molecular formula C24H18As2O5S4. Its structure is based around a fluorescein core with two substituents. It is used in bioanalytical research as a fluorescent label for visualising proteins in living cells. FlAsH-EDT2 is an abbreviation for fluorescin arsenical hairpin binder-ethanedithiol, and is a pale yellow or pinkish fluorogenic solid. It has a semi-structural formula (C2H4AsS2)2-(C13H5O3)-C6H4COOH, representing the dithiarsolane substituents bound to the hydroxyxanthone core, attached to an o-substituted molecule of benzoic acid. FlAsH-EDT2 is used for site-specific labelling, selectively binding to proteins containing the tetracysteine (TC) motif Cys-Cys-Xxx-Xxx-Cys-Cys and becoming fluorescent when bound. It displays non-specific binding to endogenous cysteine-rich proteins, meaning it binds to sites other than the one of interest (CCXXCC). Further optimization of the TC motif has revealed improved FlAsH binding affinity for a CCPGCC motif, and higher quantum yield when the tetracysteine motif is flanked with specific residues (HRWCCPGCCKTF or FLNCCPGCCMEP).c residues (HRWCCPGCCKTF or FLNCCPGCCMEP).
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| rdfs:comment |
FlAsH-EDT2 is an organoarsenic compound wi … FlAsH-EDT2 is an organoarsenic compound with molecular formula C24H18As2O5S4. Its structure is based around a fluorescein core with two substituents. It is used in bioanalytical research as a fluorescent label for visualising proteins in living cells. FlAsH-EDT2 is an abbreviation for fluorescin arsenical hairpin binder-ethanedithiol, and is a pale yellow or pinkish fluorogenic solid. It has a semi-structural formula (C2H4AsS2)2-(C13H5O3)-C6H4COOH, representing the dithiarsolane substituents bound to the hydroxyxanthone core, attached to an o-substituted molecule of benzoic acid.an o-substituted molecule of benzoic acid.
, Fluorescein arsenical helix binder, kurz F … Fluorescein arsenical helix binder, kurz FlAsH-EDT2, ist eine vom Fluoreszenzfarbstoff Fluorescein abgeleitete arsenhaltige chemische Verbindung, die in Peptiden und Proteinen selektiv an bestimmte Strukturen (sog. Tetracysteinsequenzen) binden kann. Nach Bindung an seine Zielstruktur und einer Anregung mit kurzwelligem Licht zeigt FlAsH eine Fluoreszenz. FlAsH-EDT2 ist daher als Protein-Tag zur selektiven Markierung und Identifizierung von Proteinen geeignet. Ebenso wird dieser Farbstoff als ein Fluorophor für Förster-Resonanzenergietransfers (FRET) verwendet. FlAsH-EDT2 kann sowohl in die Gruppe der Triphenylmethanfarbstoffe, als auch der Xanthenfarbstoffe eingeordnet werden. der Xanthenfarbstoffe eingeordnet werden.
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| rdfs:label |
Fluorescein Arsenical Helix Binder
, FlAsH-EDT2
|
